一、科研项目

2009年新增科研项目28项，其中国家级8项（仪器专项1项），省部级5项，具体如下。

二、科研经费

新增科研项目经费为430万元，到帐经费485万元，主要用于科研中小型设备费、材料费、测试化验加工费、燃料费、会议差旅费、国际合作交流费以及文献/出版和劳务费。

三、获奖成果和专利

谢青季教授指导的硕士论文《几个生物分析体系的压电电化学及荧光光谱法研究》获得湖南省优秀硕士学位论文。

获批专利3项，申报专利2项。

四、发表的论文

2009年重点实验室发表的论文共100多篇，SCI收录72篇。其中影响因子4以上的明显上升，尤其在Anal. Chem., J. Phys. Chem. B, Organ. Lett., J. Organ. Chem. Biosens. Bioelectron., Electrochem. Commun.，Catal. Lett. 等杂志上发表论文数量明显增加。具体发表论文见下表：

1. Cao Z., Hu Y., Wu W., Ha T., Kelley J., Deng C., Chen Q., Li C., Li J. andLi Y., The TIR/BB-loop mimetic AS-1 protects the myocardium from ischaemia/reperfusion injury,Cardiovascular Research, 2009,84, 442-451.

2. Chen C., Fu Y., Xiang C.,Xie Q., Zhang Q., Su Y., Wang L. and Yao S., Electropolymerization of preoxidized catecholamines on Prussian blue matrix to immobilize glucose oxidase for sensitive amperometric biosensing,Biosensors & Bioelectronics, 2009,24, 2726-2729.

3. Chen S., Chu Y., Zheng J. andLi Z.,Study on the two dealloying modes in the electrooxidation of Au-Sn alloys by in situ Raman spectroscopy,Electrochimica Acta, 2009,54, 1102-1108.

4. Chen S., Wu S., Zheng J. andLi Z., Spectroscopic and morphological studies on the electrooxidation of Pt-Ni alloys in HCl solution,Journal of Electroanalytical Chemistry, 2009,628, 55-59.

5. Chen X., Chen S., Huang W., Zheng J. andLi Z., Facile preparation of Bi nanoparticles by novel cathodic dispersion of bulk bismuth electrodes,Electrochimica Acta, 2009,54, 7370-7373.

6. Chen Y.-Z., Liu G.-Z., Shen Y.,Chen B. and Zeng J.-G., Analysis of alkaloids in Macleaya cordata (Willd.) R. Br. using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry,Journal of Chromatography A, 2009,1216, 2104-2110.

7. Chu Y., Chen S., Zheng J. andLi Z., Elimination of oxidation and decomposition by SnCl₂in the SERS study of pyridoxine on a roughened Au electrode,Journal of Raman Spectroscopy, 2009,40, 229-233.

8. Deng C.-L., Song R.-J., Liu Y.-L. andLi J.-H., General Zinc-Catalyzed Conia-Ene Reactions of 1,3-Dicarbonyl Compounds with Alkynes Including the Classically Challenging Substrates under Neat Conditions,Advanced Synthesis & Catalysis, 2009,351, 3096-3100.

9. Deng C.-L., Zou T., Wang Z.-Q., Song R.-J. andLi J.-H., Copper/Silver-Cocatalyzed Conia-Ene Reactions of 2-Alkynic 1,3-Dicarbonyl Compounds,Journal of Organic Chemistry, 2009,74, 412-414.

10. Deng W., Tan Y., Fang Z.,Xie Q., Li Y., Liang X. and Yao S., ABTS-Multiwalled Carbon Nanotubes Nanocomposite/Bi Film Electrode for Sensitive Determination of Cd and Pb by Differential Pulse Stripping Voltammetry,Electroanalysis, 2009,21, 2477-2485.

11. Fu Y., Li P.,Xie Q., Xu X., Lei L., Chen C., Zou C., Deng W. and Yao S., One-Pot Preparation of Polymer-Enzyme-Metallic Nanoparticle Composite Films for High-Performance Biosensing of Glucose and Galactose,Advanced Functional Materials, 2009,19, 1784-1791.

12. Fu Y., Zou C.,Xie Q., Xu X., Chen C., Deng W. and Yao S., Highly Sensitive Glucose Biosensor Based on One-Pot Biochemical Preoxidation and Electropolymerization of 2,5-Dimercapto-1,3,4-thiadiazole in Glucose Oxidase-Containing Aqueous Suspension,Journal of Physical Chemistry B, 2009,113, 1332-1340.

13. Ge B., Tan Y., Xie Q.,Ma M. and Yao S., Preparation of chitosan-dopamine-multiwalled carbon nanotubes nanocomposite for electrocatalytic oxidation and sensitive electroanalysis of NADH,Sensors and Actuators B-Chemical, 2009,137, 547-554.

14. Guo X., Chen X.-P., Cheng Z.-N., Luo X., Guo R., Chen L., Chen J.,Chen B., Peng J. and Li Y.-J., No effect of MDR1 C3435T polymorphism on oral pharmacokinetics of telmisartan in 19 healthy Chinese male subjects,Clinical Chemistry and Laboratory Medicine, 2009,47, 38-43.

15. Guo Y.-J., Tang R.-Y.,Li J.-H., Zhong P. and Zhang X.-G., Palladium-Catalyzed Annulation of 2-(1-Alkynyl)benzenamines with Disulfides: Synthesis of 3-Sulfenylindoles,Advanced Synthesis & Catalysis, 2009,351, 2615-2618.

16. He D.,Chen B., Tian Q. and Yao S., Simultaneous determination of five anthraquinones in medicinal plants and pharmaceutical preparations by HPLC with fluorescence detection,Journal of Pharmaceutical and Biomedical Analysis, 2009,49, 1123-1127.

17. Hong Y.-J., Li T.-Y.,Chen B. and Yao S.-Z., Immobilization of Angiotensin Converting-enzyme on Chitosan Microspheres,Chemical Journal of Chinese Universities-Chinese, 2009,30, 328-331.

18. Huang J.,Xie Q., Tan Y., Fu Y., Su Z., Huang Y. and Yao S., Preparation of Pt/multiwalled carbon nanotubes modified Au electrodes via Pt-Cu co-electrodeposition/Cu stripping protocol for high-performance electrocatalytic oxidation of methanol,Materials Chemistry and Physics, 2009,118, 371-378.

19. Huang M.-Z., Luo F.-X., Mo H.-B., Ren Y.-G., Wang X.-G., Ou X.-M., Lei M.-X., Liu A.-P., Huang L. andXu M.-C., Synthesis and Herbicidal Activity of Isoindoline-1,3-dione Substituted Benzoxazinone Derivatives Containing a Carboxylic Ester Group,Journal of Agricultural and Food Chemistry, 2009,57, 9585-9592.

20. Huang W., Chen S., Zheng J. andLi Z., Facile preparation of Pt hydrosols by dispersing bulk Pt with potential perturbations,Electrochemistry Communications, 2009,11, 469-472.

21. Huang W., Wang M., Zheng J. andLi Z., Facile Fabrication of Multifunctional Three-Dimensional Hierarchical Porous Gold Films via Surface Rebuilding,Journal of Physical Chemistry C, 2009,113, 1800-1805.

22. Huang X.-C., Wang F., Liang Y. andLi J.-H., Halopalladation/Decarboxylation/Carbon - Carbon Forming Domino Process: Synthesis of 5-Halo-6-substituted Benzo b naphtho 2,1-d furans,Organic Letters, 2009,11, 1139-1142.

23. Jia X., Tan L., Zhou Y., Jiang X.,Xie Q., Tang H. and Yao S., Magnetic immobilization and electrochemical detection of leukemia K562 cells,Electrochemistry Communications, 2009,11, 141-144.

24. Jia X., Zhou Y., Tan L.,Xie Q., Tang H., Ma M. and Yao S., Hydroxyapitite-multiwalled carbon nanotubes nanocomposite for adhesion and electrochemical study of human osteoblast-like cells (MG-63),Electrochimica Acta, 2009,54, 3611-3617.

25. Jiang T.-S. andLi J.-H., Palladium-catalyzed oxidative tandem reaction of allylamines with aryl halides leading to alpha,beta-unsaturated aldehydes,Chemical Communications, 2009, 7236-7238.

26. Lei L., Fu Y., Xu X.,Xie Q. and Yao S., Aptamer-Based Electrochemical Biosensors,Progress in Chemistry, 2009,21, 724-731.

27. Li T.-Y., Deng K.-G.,Chen B. and Yao S.-Z., Determination of lipase activity by gas chromatography,Yao xue xue bao = Acta pharmaceutica Sinica, 2009,44, 628-631.

28. Li X., Jia X., Xie C., Lin Y., Cao R., He Q., Chen P., Wang X. andLiang S., Development of cationic colloidal silica-coated magnetic nanospheres for highly selective and rapid enrichment of plasma membrane fractions for proteomics analysis,Biotechnology and Applied Biochemistry, 2009,54, 213-220.

29. Liu A., He H., Liu X., He L., Hu C., Wei Z., Hu Z., Huang M., Ou X. andSu S., Synthesis and Fungicidal Activities of Novel Oxime Ether Strobilurins,Chinese Journal of Organic Chemistry, 2009,29, 1381-1386.

30. Liu G., Ma J., Chen Y., Tian Q., Shen Y., Wang X.,Chen B. and Yao S., Investigation of flavonoid profile of Scutellaria bacalensis Georgi by high performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry,Journal of Chromatography A, 2009,1216, 4809-4814.

31. Liu G., Xu Z., Chen J., Lang G., Tian Q., Shen Y.,Chen B. and Yao S., On-line strategies for the identification of unknown flavone glycosides in Dracocephalum tanguticum Maxim,Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2009,877, 2545-2550.

32. Liu J., Huang W., Chen S., Hu S., Liu F. a. andLi Z., Facile Electrochemical Dispersion of Bulk Rh into Hydrosols,International Journal of Electrochemical Science, 2009,4, 1302-1308.

33. Liu P., Zhang Q., He L.,Xie Q. and Ding H., Synthesis and Properties of Poly(Urethane-Imide) Diacid/Epoxy Composites Cured with an Aziridine System,Journal of Applied Polymer Science, 2009,113, 2628-2637.

34. Liu Y.-L., Liang Y., Pi S.-F., Huang X.-C. andLi J.-H., Palladium-Catalyzed Cocyclotrimerization of Allenes with Arynes: Selective Synthesis of Phenanthrenes,Journal of Organic Chemistry, 2009,74, 3199-3202.

35. Liu Y.-L., Liang Y., Pi S.-F. andLi J.-H., Selective Synthesis of o-Acylbenzylphosphonates by Insertion Reactions of Arynes into beta-Ketophosphonates,Journal of Organic Chemistry, 2009,74, 5691-5694.

36. Lu Y., Chen J.,Chen B. and Yao S., Simultaneous determination of 8 illegal synthetic drugs in antihypertensive dietary supplements by high performance liquid chromatography-mass spectrometry,Se pu = Chinese journal of chromatography / Zhongguo hua xue hui, 2009,27, 44-49.

37. Luo P., Tang R., Zhong P. andLi J., Progress in the Synthesis of Nitrogen-Containing Heterocycles by Intramolecular Cyclization of Alkynes,Chinese Journal of Organic Chemistry, 2009,29, 1924-1937.

38. Luo P.-S., Wang F.,Li J.-H., Tang R.-Y. and Zhong P., Copper-Catalyzed Selective S-Arylation of 1,2-Bis(o-amino-1H-pyrazolyl) Disulfides with Arylboronic Acids,Synthesis-Stuttgart, 2009, 921-928.

39. Luo P.-S., Yu M., Tang R.-Y., Zhong P. andLi J.-H., Solvent-free copper-catalyzed oxidative S-arylation of 1,2-diaryldisulfides with aryltrimethoxysilane,Tetrahedron Letters, 2009,50, 1066-1070.

40. Peng P., Tang B.-X., Pi S.-F., Liang Y. andLi J. H., Synthesis of (E)-3-(Isobenzofuran-3(1H)-ylidene)-indolin-2-ones by the Palladium-Catalyzed Intramolecular C-H Functionalization Process,Journal of Organic Chemistry, 2009,74, 3569-3572.

41. Peng X., Ma J., Cheng K.-W.,Chen B., Chen F. and Wang M., High-performance liquid chromatographic determination of creatine kinase activity influenced by methylglyoxal,Biomedical Chromatography, 2009,23, 170-174.

42. Pi S.-F., Tang B.-X., Li J.-H., Liu Y.-L. andLiang Y., Palladium-Catalyzed Decarboxylative Coupling of Allylic Alkynoates with Arynes,Organic Letters, 2009,11, 2309-2312.

43. Qiu J.-W., Zhang X.-G., Tang R.-Y., Zhong P. andLi J.-H., Iron-Catalyzed Tandem Reactions of 2-Halobenzenamines with Isothiocyanates Leading to 2-Aminobenzothiazoles,Advanced Synthesis & Catalysis, 2009,351, 2319-2323.

44. Shen Y., Hu Y., Huang K., Yin S. a.,Chen B. and Yao S., Solid-phase extraction of carotenoids,Journal of Chromatography A, 2009,1216, 5763-5768.

45. Song R.-J., Liu Y., Li R.-J. andLi J.-H., Selective synthesis of 3-methyleneindolin-2-ones by one-pot multicatalytic processes,Tetrahedron Letters, 2009,50, 3912-3916.

46. Su Z., Huang J.,Xie Q., Fang Z., Zhou C., Zhou Q. and Yao S., Electrochemical quartz crystal microbalance study of covalent tethering of carboxylated thiol to polyaniline for electrocatalyzed oxidation of ascorbic acid in neutral aqueous solution,Physical Chemistry Chemical Physics, 2009,11, 9050-9061.

47. Tan L., Jia X. e., Jiang X., Zhang Y., Tang H., Yao S. andXie Q., In vitro study on the individual and synergistic cytotoxicity of adriamycin and selenium nanoparticles against Bel7402 cells with a quartz crystal microbalance,Biosensors & Bioelectronics, 2009,24, 2268-2272.

48. Tan L., Xie Q., jia X. e., Guo M.,Zhang Y., Tang H. and Yao S., Dynamic measurement of the surface stress induced by the attachment and growth of cells on Au electrode with a quartz crystal microbalance,Biosensors & Bioelectronics, 2009,24, 1603-1609.

49. Tan R., Yin D., Yu N., Zhao H. andYin D., Easily recyclable polymeric ionic liquid-functionalized chiral salen Mn(III) complex for enantioselective epoxidation of styrene,Journal of Catalysis, 2009,263, 284-291.

50. Tan Y., Deng W., Ge B.,Xie Q., Huang J. and Yao S., Biofuel cell and phenolic biosensor based on acid-resistant laccase-glutaraldehyde functionalized chitosan-multiwalled carbon nanotubes nanocomposite film,Biosensors & Bioelectronics, 2009,24, 2225-2231.

51. Tang D.-J., Tang B.-X. andLi J.-H., Selective Synthesis of 3-Aryl Quinolin-2(1H)-ones and 3-(1-Arylmethylene)oxindoles Involving a 2-Fold Arene C-H Activation Process,Journal of Organic Chemistry, 2009,74, 6749-6755.

52. Tang F., Zhang Q., Nie Z.,Chen B. and Yao S., Sample preparation for analyzing traditional Chinese medicines,Trac-Trends in Analytical Chemistry, 2009,28, 1253-1262.

53. Tao L., Xie Y., Deng C. andLi J., Generation of Pd Nanoparticles in situ from PdCl₂in TBAF: An Efficient and Reusable Catalytic System for the Suzuki-Miyaura Reaction under Ligand- and Solvent-free Conditions,Chinese Journal of Chemistry, 2009,27, 1365-1373.

54. Tetala K. K. R., Swarts J. W.,Chen B., Janssen A. E. M. and van Beek T. A., A three-phase microfluidic chip for rapid sample clean-up of alkaloids from plant extracts,Lab on a Chip, 2009,9, 2085-2092.

55. Tu X., Yan L., Luo X., Luo S. andXie Q., Electroanalysis of Bisphenol A at a Multiwalled Carbon Nanotubes-gold Nanoparticles Modified Glassy Carbon Electrode,Electroanalysis, 2009,21, 2491-2494.

56. Wang J., Yang D., Wang Z.,Chen B.and Yao S., Simultaneous determination of illegal additives in dietary supplements and traditional medicines by high performance liquid chromatography-electrospray ionization mass spectrometry (vol 113, pg 227, 2009),Food Chemistry, 2009,114, 763-763.

57. Wang J., Yang D., Wang Z.,Chen B. and Yao S., Simultaneous of illegal additives in dietary supplements and traditional medicines by high performance liquid chromatography-electrospray ionization mass spectrometry,Food Chemistry, 2009,113, 227-232.

58. Wang Z.-L., Tang R.-Y., Luo P.-S., Deng C.-L., Zhong P. andLi J.-H., Hydrothiolation of terminal alkynes with diaryl disulfides and diphenyl diselenide: selective synthesis of (Z)-1-alkenyl sulfides and selenides,Tetrahedron, 2009,65, 579-579.

59. Wang Z.-Q., Liang Y., Lei Y., Zhou M.-B. andLi J.-H., Iron-catalyzed annulations of 2-(2-alkynyl)phenoxy)-1-arylethanones leading to substituted naphthalen-1-ols,Chemical Communications, 2009, 5242-5244.

60. Wang Z.-Q., Tang B.-X., Zhang H.-P., Wang F. andLi J.-H., Electrophilic ipso-Halocyclization of N-Arylpropynamides with Polyfluoroalkyl Alcohols: Selective Synthesis of 8-(Polyfluoroalkoxy)azaspiro 4.5 trienes,Synthesis-Stuttgart, 2009, 891-902.

61. Wu D.-X., Luo F.-X., Mo H.-B., Wang X.-G., Ren Y.-G., Simpson J. andHuang M.-Z., Synthesis and Crystal Structure of Ethyl 2-(6-(1,3-Dioxo-4,5,6,7-tetrahydro-1H-isoindol-2(3H)-yl)-7-fluoro-3-oxo- 2H-benzo b 1,4 oxazin-4(3H)-yl) Butanoate,Chinese Journal of Structural Chemistry, 2009,28, 585-589.

62. Xie F., Fu Z., Zhong S., Ye Z., Zhou X., Liu F., Lu C., Rong C., Mao L. andYin D., Catalytic Oxidative Removal of Dimethylsulfoxide by Hexadentate 8-Quinolinolato Manganese(III) Complexes,Chinese Journal of Catalysis, 2009,30, 981-985.

63. Xie F., Fu Z., Zhong S., Ye Z., Zhou X., Liu F., Rong C., Mao L. andYin D., Thioanisole oxidation with hydrogen peroxide catalyzed by hexadentate 8-quinolinolato manganese(III) complexes,Journal of Molecular Catalysis a-Chemical, 2009,307, 93-97.

64. Xu X., Nie Z., Chen J., Fu Y., Li W., Shen Q. andYao S., A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase,Chemical Communications, 2009, 6946-6948.

65. Yang D.-j. andChen B., Simultaneous Determination of Nonnutritive Sweeteners in Foods by HPLC/ESI-MS,Journal of Agricultural and Food Chemistry, 2009,57, 3022-3027.

66. Ye M., Zhang Y., Li H.,Zhang Y., Tan P., Tang H. and Yao S., A novel method for the detection of point mutation in DNA using single-base-coded CdS nanoprobes,Biosensors & Bioelectronics, 2009,24, 2339-2345.

67. Ye Z., Fu Z., Zhong S., Xie F., Zhou X., Liu F. andYin D., Selective oxidation of alcohols with hydrogen peroxide catalyzed by hexadentate binding 8-quinolinolato manganese(III) complexes,Journal of Catalysis, 2009,261, 110-115.

68. Yu M., Tang R.-Y. andLi J.-H., Synthesis of 6,7-dihydro-5H-dibenzo c,e azepines and biaryls by palladium-catalyzed Ullmann reaction,Tetrahedron, 2009,65, 3409-3416.

69. Yu R., Ma M., Wang L.,Xie Q., Cao Z., Jiang X. and Yao S., Highly sensitive and surface-renewable electrochemical quartz crystal microbalance assays of heparin and chondroitin sulfate based on their effects on the electrodeposition of neutral red,Biosensors & Bioelectronics, 2009,24, 1771-1776.

70. Zhang Q., Chen C.,Xie Q.and Liu P., Electrodeposition of a biocompatible hydroxyapatite matrix to immobilize glucose oxidase for sensitive glucose biosensing,Microchimica Acta, 2009,165, 223-229.

71. Zhu L., Ding L., Zhang Q., Wang L., Tang F., Liu Q. andYao S., Direct analysis of cryptotanshinone and tanshinone IIA in biological samples and herbal medicinal preparations by a green technique of micellar liquid chromatography,Green Chemistry, 2009,11, 132-137.

72. Zou T., Pi S.-S. andLi J.-H., FeCl₃-Catalyzed 1,2-Addition Reactions of Aryl Aldehydes with Arylboronic Acids,Organic Letters, 2009,11, 453-456.

五、人才引进和研究生培养

队伍建设和人才培养呈现出良好的发展态势，2009年新进博士2名，新晋教授1人。培养博士毕业研究生9人，硕士毕业研究生50人。新招收博士7人，硕士96人。

六、国内外学术交流和会议

2009年学术氛围异常活跃，邀请国内外知名教授来院来室作学术报告和学术交流，主要情况如下：

七、依托单位给予的支持

学校按照教育部重点实验室建设要求和管理条例，在人力、物力和财力方面对“化学生物学及中药分析”重点实验室给予全力支持,学校每年20万元的运行经费。

八、运行经费的使用情况

2009年学校拨运行经费20万，主要用于开放基金、学术委员会开支，仪器设备费、材料与资料费、会议差旅费、维修费、学术交流、复印与打印等方面。

另外，通过重点学科和湖南省拨给姚院士的经费，投入实验室购置紫外分光光度计等仪器设备。

七、开放基金

本实验室已设立开放基金，经学术委员会讨论，2009年批准12项。