《教育部重点实验室工作年报》

一、实验室名称，学科（领域），依托单位

实验室名称：化学生物学及中药分析

Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research

主管部门：湖南省教育厅

学科领域：化学

依托单位：湖南师范大学

主 管 部 门： 湖南省教育厅

二、实验室工作纪要

“化学生物学及中药分析”教育部重点实验室由中科院院士姚守拙教授领衔，拥有一批以中青年教授、博士为主体的学术队伍。实验室是在2004年4月通过教育部论证评估，被列为教育部重点实验室建设基地，2006年6月22日通过教育部验收正式运行。“十一五”以来，实验室根据湖南地区中草药资源丰富的特点，将中药研究与新兴学科化学生物学相结合，开展中药分析与化学生物学研究、化学生物学中的新分析方法、活性小分子的合成与化学生物作用、毒物化学生物学研究等方向的研究，获得了一批高水平的基础理论研究和应用研究成果。实验室研发工作基本思路如下：体现化学、生物和药学等学科交叉融合、相互渗透的理念，运用化学的理论、方法、手段乃至研究的思维，去研究和解决中药及化学生物学的问题，加速推进中药学和生命科学研究的发展；利用生物学的原理、方法、技术以及新发现、新成果解决化学领域中的一些难题，以此促进化学与生命科学的融合，推动新型学科化学生物学向更高层次发展。以分离分析、合成和构效关系模拟计算等为基本手段，探索和了解小分子与生物大分子作用过程、机制和效应，延伸到现代中药、新药研发、生态农药、绿色农业、重大疾病早期预警等应用领域，为社会经济发展服务。

2010年来，共承担了30余项研究课题，在研总经费1420万元，其中2011年新增科研课题28项，其中国家级10项，总经费607.4万元。实验室已具有承担国家重大、重点课题的能力。2010年发表99篇学术论文，获专利授权4项。对于实验室的发展而言，2010年是值得肯定的一年，经过科研工作者和全体工作人员的共同努力，重点实验室取得长足的进步，各研究室在科学研究、人才培养、学术交流、运行管理和硬件设备建设等方面都做出了很大的努力，取得了很好的成绩，为实验室的发展增添了新的色彩。

1. 科研项目

目前共承担的在研科研课题60余项，其中2010年新增科研项目30项。其中国家级10项（包括国家自然科学基金9项和国家863课题课题1项）。具体新增课题情况如下表：

2、科研经费

新增科研经费为621万元，主要用于科研中小型设备费、材料费、测试化验加工费、燃料费、会议差旅费、国际合作交流费以及文献/出版和劳务费。

2010年， 在研项目经费1200多万元，在研的部分科研项目如下表：

3. 获奖成果

4、发表的论文

2010年， 实验室共发表90多篇，其中SCI收录62篇， 在J. Am. Chem. Soc.，Anal. Chem.等知名杂志和影响因子4以上发表20多篇。具体见后。

5、获批专利

6、人才引进和研究生培养

2010年，实验室引进博士3人：傅迎春，赵海红，孟勇，分别在分析化学、有机化学和化学工程等方面胜任教学和科研工作。

2010年共招生硕士研究生109人，博士研究生8人，毕业博士研究生8人，硕士研究生63人。

7、访问学者

实验室唐浩、张友玉、喻宁亚、梁云老师等都在国内外知名大学进行访问学者研究学习。

三、学术委员会会议纪要

2010年未召开学术委员会。

四、国内外学术交流和会议

2010年，实验室学术氛围异常活跃，实验室人员参加国内外会议20多场次。实验室人员邀请国内外知名教授来院来室作学术报告和学术交流9场（具体如下表）。

2010年实验室在湖南师大国际学术交流合作中心召开中-日分析仪器研发学术交流会及湖南师范大学-岛津合作实验室挂牌仪式，匡乐满副校长、姚守拙院士和日本岛津公司社长参加揭牌仪式。8月27-29日召开国家自然科学基金委化学分析部分析化学学科2010年度“仪器专项”项目汇报与交流会。会议对目前仪器分析与装置的发展现状及发展前沿进行了深入地研讨。

五、发表的主要学术论文

实验室发表的论文如下：

1. Chen B.,Ma M. and Su X., An amperometric penicillin biosensor with enhanced sensitivity based on co-immobilization of carbon nanotubes, hematein, and beta-lactamase on glassy carbon electrode,Analytica Chimica Acta, 2010,674, 89-95.

2. Deng G.and Zou J., The first nucleophilic substitution reaction of organoindium reagents with sulfonyl chlorides: a facile method for preparation of vinyl sulfones,Arkivoc, 2010, 186-194.

3. Deng W., Tan Y., Li Y., Wen Y., Su Z., Huang Z., Huang S., Meng Y.,Xie Q., Luo Y. and Yao S., Square wave voltammetric determination of Hg(II) using thiol functionalized chitosan-multiwalled carbon nanotubes nanocomposite film electrode,Microchimica Acta, 2010,169, 367-373.

4. Dong S.-X., Zhang X.-G., Liu Q., Tang R.-Y., Zhong P. andLi J.-H., Synthesis of 1-Substituted 2-(Trifluoromethyl)indoles via a Palladium-Catalyzed Double Amination Reaction,Synthesis-Stuttgart, 2010, 1521-1525.

5. Fu Y., Li P., Bu L., Wang T.,Xie Q., Xu X., Lei L., Zou C. and Yao S., Chemical/Biochemical Preparation of New Polymeric Bionanocomposites with Enzyme Labels Immobilized at High Load and Activity for High-Performance Electrochemical Immunoassay,Journal of Physical Chemistry C, 2010,114, 1472-1480.

6. Fu Y., Li P., Wang T., Bu L.,Xie Q., Xu X., Lei L., Zou C., Chen J. and Yao S., Novel polymeric bionanocomposites with catalytic Pt nanoparticles label immobilized for high performance amperometric immunoassay,Biosensors & Bioelectronics, 2010,25, 1699-1704.

7. Guo B., Duan J.-M., Wang M.-L., Wang X.-Y. andChen B., Flow Injection-Electrospray Ionization Mass Spectrometry Based High-Throughput Inhibition Screening of Herbal Extracts Toward Major Human Cytochrome P450s,Chinese Journal of Analytical Chemistry, 2010,38, 1692-1697.

8. Guo B., Huang Z., Wang M., Wang X., Zhang Y.,Chen B., Li Y., Yan H. and Yao S., Simultaneous direct analysis of benzimidazole fungicides and relevant metabolites in agricultural products based on multifunction dispersive solid-phase extraction and liquid chromatography-mass spectrometry,Journal of Chromatography A, 2010,1217, 4796-4807.

9. Guo Y.-J., Tang R.-Y., Zhong P. andLi J.-H., Copper-catalyzed tandem reactions of 2-halobenzenamines with isothiocyanates under ligand- and base-free conditions,Tetrahedron Letters, 2010,51, 649-652.

10. Hu S., Huang W. andLi Z., Facile fabrication of 3D dendritic gold nanostructures with an AuSn alloy by square wave potential pulse,Materials Letters, 2010,64, 1257-1260.

11. Huang W., Fu L., Yang Y., Hu S., Li C. andLi Z., Simultaneous Fabrications of Nanoparticles and 3D Porous Films of Sn or Pb from Pure Electrodes,Electrochemical and Solid State Letters, 2010,13, K46-K48.

12. Huang Z., Jia X. E.,Xie Q., Tan Y., Huang S., Huang J., Meng Y. and Yao S., Electrochemical quartz crystal microbalance study on Au-supported Pt adlayers for electrocatalytic oxidation of methanol in alkaline solution,Science China-Chemistry, 2010,53, 2349-2356.

13. Jiang X., Tan L., Zhang B., Zhang Y., Tang M.,Xie Q. and Yao S., Detection of adherent cells using electrochemical impedance spectroscopy based on molecular recognition of integrin beta 1,Sensors and Actuators B-Chemical, 2010,149, 87-93.

14. Li C.-L., Zhang X.-G., Fang R.-Y., Zhong P. andLi J.-H., Copper-Catalyzed Thiolation Annulations of 1,4-Dihalides with Sulfides Leading to 2-Trifluoromethyl Benzothiophenes and Benzothiazoles,Journal of Organic Chemistry, 2010,75, 7037-7040.

15. Li O., Su X., He D., Chen Y., Ma M.,Xie Q. and Yao S., A study on separation and extraction of four main alkaloids in Macleaya cordata (Willd) R. Br. with strip dispersion hybrid liquid membrane,Journal of Separation Science, 2010,33, 2026-2034.

16. Li R.-J., Pi S.-F., Liang Y., Wang Z.-Q., Song R.-J., Chen G.-X. andLi J.-H., Palladium-catalyzed annulations of arynes with 2-(2-iodophenoxy)-1-substituted ethanones,Chemical Communications, 2010,46, 8183-8185.

17. Li Y., Tan Y., Deng W.,Xie Q., Zhang Y., Chen J. and Yao S., Electropolymerization of catecholamines after laccase-catalyzed preoxidation to efficiently immobilize glucose oxidase for sensitive amperometric biosensing,Sensors and Actuators B-Chemical, 2010,151, 30-38.

18. Lian S., Qi Y., Rong C., Yu L., Zhu A.,Yin D. and Liu S., Effectively Leveraging Solar Energy through Persistent Dual Red Phosphorescence: Preparation, Characterization, and Density Functional Theory Study of Ca₂Zn₄Ti₁₆O₃₈:Pr³⁺,Journal of Physical Chemistry C, 2010,114, 7196-7204.

19. Liu F., Fu Z., Liu Y., Lu C., Wu Y., Xie F., Ye Z., Zhou X. andYin D., A Simple and Environmentally Benign Method for Sulfoxidation of Sulfides with Hydrogen Peroxide,Industrial & Engineering Chemistry Research, 2010,49, 2533-2536.

20. Liu G., Zhou N., Zhang M., Li S., Tian Q., Chen J.,Chen B., Wu Y. and Yao S., Hydrophobic solvent induced phase transition extraction to extract drugs from plasma for high performance liquid chromatography-mass spectrometric analysis,Journal of Chromatography A, 2010,1217, 243-249.

21. Liu P., Zhang Q., He L.,Xie Q. and Ding H., Thermal and Mechanical Properties of Poly(urethane-imide)/Epoxy/Silica Hybrids,Journal of Applied Polymer Science, 2010,117, 3722-3728.

22. Liu Y., Song R.-J. andLi J.-H., Yb(OTf)(3)-Promoted ZnCl₂-Catalyzed Conia-Ene Reaction of Linear beta-Alkynic beta-Dicarbonyls,Synthesis-Stuttgart, 2010, 3663-3669.

23. Lu C., Fu Z., Liu Y., Liu F., Wu Y., Qin J., He X. andYin D., A moderate and efficient method for oxidation of ethylbenzene with hydrogen peroxide catalyzed by 8-quinolinolato manganese(III) complexes,Journal of Molecular Catalysis a-Chemical, 2010,331, 106-111.

24. Lu C.-L., Fu Z.-H., Liu Y.-C., Liu F.-L., Qin J.-W., He X.-L. andYin D.-L., Selective Oxidation of Ethylbenzene with Hydrogen Peroxide Catalyzed by 5,7-Dibromo-8-quinolinolato Manganese Complexes,Acta Physico-Chimica Sinica, 2010,26, 2705-2710.

25. Lu Y. L., Zhou N. L., Liao S. Y., Su N., He D. X., Tian Q. Q.,Chen B. and Yao S. Z., Detection of adulteration of anti-hypertension dietary supplements and traditional Chinese medicines with synthetic drugs using LC/MS,Food Additives and Contaminants Part a-Chemistry Analysis Control Exposure & Risk Assessment, 2010,27, 893-902.

26. Luo J., Liu M., Zhao Q., Zhao J., Zhang Y., Tan L., Tang H.,Xie Q., Li H. and Yao S., A study on the electro-oxidation and electropolymerization of a new OPE linear molecule by EQCM and in situ FTIR spectroelectrochemistry,Electrochimica Acta, 2010,56, 454-462.

27. Luo X., Jiang X., Tu X., Luo S., Yan L. andChen B., Determination of malachite green in fish water samples by cloud-point extraction coupled to cation-selective exhaustive injection and sweeping-MEKC,Electrophoresis, 2010,31, 688-694.

28. Ouyang L., Su X., He D., Chen Y., Ma M.,Xie Q. and Yao S., A study on separation and extraction of four main alkaloids in Macleaya cordata (Willd) R. Br. with strip dispersion hybrid liquid membrane,Journal of Separation Science, 2010,33, 2026-2034.

29. Pi S.-F., Yang X.-H., Huang X.-C., Liang Y., Yang G.-N., Zhang X.-H. andLi J.-H., Palladium-Catalyzed Cyclocarbonylation of Arynes with Methyl Allyl Carbonates: Selective Synthesis of 1H-Inden-1-ones,Journal of Organic Chemistry, 2010,75, 3484-3487.

30. Shen Y., Hu Y.,Chen B. and Yao S., Screening of enzyme inhibitors from traditional Chinese medicine,Combinatorial chemistry & high throughput screening, 2010,13, 885-899.

31. Shi G., Rao L.,Xie Q., Li J., Li B. and Xiong X., Characterization of yeast cells as a microencapsulation wall material by Fourier-transform infrared spectroscopy,Vibrational Spectroscopy, 2010,53, 289-295.

32. Tan Y., Deng W., Chen C.,Xie Q., Lei L., Li Y., Fang Z., Ma M., Chen J. and Yao S., Immobilization of enzymes at high load/activity by aqueous electrodeposition of enzyme-tethered chitosan for highly sensitive amperometric biosensing,Biosensors & Bioelectronics, 2010,25, 2644-2650.

33. Tan Y., Deng W., Li Y., Huang Z., Meng Y.,Xie Q., Ma M. and Yao S., Polymeric Bionanocomposite Cast Thin Films with In Situ Laccase-Catalyzed Polymerization of Dopamine for Biosensing and Biofuel Cell Applications,Journal of Physical Chemistry B, 2010,114, 5016-5024.

34. Tang B.-X., Song R.-J., Wu C.-Y., Liu Y., Zhou M.-B., Wei W.-T., Deng G.-B.,Yin D.-L. and Li J.-H., Copper-Catalyzed Intramolecular C H Oxidation/Acylation of Formyl-N-arylformamides Leading to Indoline-2,3-diones,Journal of the American Chemical Society, 2010,132, 8900-8905.

35. Tang J.-S., Xie Y.-X., Wang Z.-Q., Deng C.-L. andLi J.-H., Phosphazene Base-Catalyzed Intramolecular Cascade Reactions of Aryl-Substituted Enynes,Synthesis-Stuttgart, 2010, 3204-3210.

36. Tang R.-Y. andLi J.-H., PdCl₂-Catalyzed Domino Reactions of 2-Alkynylbenzaldehydes with Indoles: Synthesis of Fluorescent 5H-Benzo b carbazol-6-yl Ketones,Chemistry-a European Journal, 2010,16, 4733-4738.

37. Tu X., Luo X., Luo S., Yan L., Zhang F. andXie Q., Novel carboxylation treatment and characterization of multiwalled carbon nanotubes for simultaneous sensitive determination of adenine and guanine in DNA,Microchimica Acta, 2010,169, 33-40.

38. Wang F., Tang B., Xie Y. andLi J., Synthesis of Alkyl-Aryl Ethers by Copper-catalyzed Etherization Reactions of Aryl Fluorides with Tetraalkylammonium Bromides and H₂O,Chinese Journal of Chemistry, 2010,28, 2318-2322.

39. Wang L., Jia X., Zhou Y.,Xie Q. and Yao S., Sandwich-type amperometric immunosensor for human immunoglobulin G using antibody-adsorbed Au/SiO2 nanoparticles,Microchimica Acta, 2010,168, 245-251.

40. Wang M., Chen S., Xia Y., Zhang Y., Huang W., Zheng J. andLi Z., Nanoassemblies of Colloidal Gold Nanoparticles by Oxygen-Induced Inorganic Ligand Replacement,Langmuir, 2010,26, 9351-9356.

41. Wang M., Guo B., Huang Z., Duan J., Chen Z.,Chen B. and Yao S., Improved compatibility of liquid chromatography with electrospray tandem mass spectrometry for tracing occurrence of barbital homologous residues in animal tissues,Journal of Chromatography A, 2010,1217, 2821-2831.

42. Wang M.-h., Chu Y.-p., Zheng J.-f. andLi Z.-l., SERS Spectra of L-Carnosine under the Interaction of Coordination with Adsorbed SnCl2,Spectroscopy and Spectral Analysis, 2010,30, 967-970.

43. Wu M., Chen Y., Wu R. a., Li R., Zou H.,Chen B. and Yao S., The synthesis of chloropropyl-functionalized silica hybrid monolithic column with modification of N,N-dimethyl-N-dodecylamine for capillary electrochromatography separation,Journal of Chromatography A, 2010,1217, 4389-4394.

44. Wu Y., Fu Z., Yin D., Xu Q., Liu F., Lu C. andMao L., Microwave-assisted hydrolysis of crystalline cellulose catalyzed by biomass char sulfonic acids,Green Chemistry, 2010,12, 696-700.

45. Xia J., Zhou N., Liu Y.,Chen B., Wu Y. and Yao S., Simultaneous determination of melamine and related compounds by capillary zone electrophoresis,Food Control, 2010,21, 912-918.

46. Xia J., Zhou N., Zhou C.,Chen B., Wu Y. and Yao S., Simultaneous determination of melamine and related compounds by hydrophilic interaction liquid chromatography-electrospray mass spectrometry,Journal of Separation Science, 2010,33, 2688-2697.

47. Xiao Z. andHe H., Synthesis, Characterization, and Catalytic Behavior of Biheteroatomic Fe-V-beta Zeolite,Chinese Journal of Catalysis, 2010,31, 705-710.

48. Yang D. andChen B., Determination of neotame in beverages, cakes and preserved fruits by column-switching high-performance liquid chromatography,Food Additives and Contaminants Part a-Chemistry Analysis Control Exposure & Risk Assessment, 2010,27, 1221-1225.

49. Yang Q., Zhang Y., Li H.,Zhang Y., Liu M., Luo J., Tan L., Tang H. and Yao S., Electrochemical copolymerization study of o-toluidine and o-aminophenol by the simultaneous EQCM and in situ FTIR spectroelectrochemisty,Talanta, 2010,81, 664-672.

50. Yu R., Wang L.,Xie Q. and Yao S., High-Performance Amperometric Sensors Using Catalytic Platinum Nanoparticles-Thionine-Multiwalled Carbon Nanotubes Nanocomposite,Electroanalysis, 2010,22, 2856-2861.

51. Zeng Y., Qian H., Chen X., Li Z., Yu S. andXiao X., Thermodynamic Estimate of pK(a) Values of the Carboxylic Acids in Aqueous Solution with the Density Functional Theory,Chinese Journal of Chemistry, 2010,28, 727-733.

52. Zhang B., Chen Q., Tang H., Xie Q., Ma M., Tan L.,Zhang Y. and Yao S., Characterization of and biomolecule immobilization on the biocompatible multi-walled carbon nanotubes generated by functionalization with polyamidoamine dendrimers,Colloids and Surfaces B-Biointerfaces, 2010,80, 18-25.

53. Zhang J., Song C.,Chen B. and Yao S., Simultaneous determination of five nucleotides in Bulbus Fritillariae by RP-HPLC,Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2010,35, 67-70.

54. Zhang L., Su X., Zhang C., Ouyang L.,Xie Q., Ma M. and Yao S., Extraction and preconcentration of beta-blockers in human urine for analysis with high performance liquid chromatography by means of carrier-mediated liquid phase microextraction,Talanta, 2010,82, 984-992.

55. Zhang M.-S., Liu G.-Z., Li S.-J., Chen B. andYao S.-Z., Solvent Induced Phase Transition Extraction Method for High Performance Liquid Chromatography-Mass Spectrometry Analysis of Plasma Samples of Pretreatment,Chemical Journal of Chinese Universities-Chinese, 2010,31, 1517-1521.

56. Zhang W.-W., Zhang X.-G. andLi J.-H., Palladium-Catalyzed Decarboxylative Coupling of Alkynyl Carboxylic Acids with Benzyl Halides or Aryl Halides,Journal of Organic Chemistry, 2010,75, 5259-5264.

57. Zhang Y., Tang Z., Wang J., Wu H., Maham A. andLin Y., Hairpin DNA Switch for Ultrasensitive Spectrophotometric Detection of DNA Hybridization Based on Gold Nanoparticles and Enzyme Signal Amplification,Analytical Chemistry, 2010,82, 6440-6446.

58. Zhang Y.-R., Wang M.-H., Zheng J.-F. andLi Z.-L., Facile Preparation of Network Thin Films of Pd Nanoparticles at the Air-Water Interface for Electrocatalysis,International Journal of Electrochemical Science, 2010,5, 1605-1611.

59. Zhao X., Zhang X.-G., Tang R.-Y., Deng C.-L. andLi J.-H., ZnI₂-Catalyzed Benzannulation of o-Alkynylbenzaldehydes with Alkenes Leading to 1-Acyl-2-Substituted Naphthalenes,European Journal of Organic Chemistry, 2010, 4211-4217.

60. Zhou M.-B., Wei W.-T., Xie Y.-X., Lei Y. andLi J.-H., Palladium-Catalyzed Cross-Coupling of Electron-Poor Terminal Alkynes with Arylboronic Acids under Ligand-Free and Aerobic Conditions,Journal of Organic Chemistry, 2010,75, 5635-5642.

61. Zhou Y., Jia X. e., Tan L.,Xie Q., Lei L. and Yao S., Magnetically enhanced cytotoxicity of paramagnetic selenium-ferroferric oxide nanocomposites on human osteoblast-like MG-63 cells,Biosensors & Bioelectronics, 2010,25, 1116-1121.

62. Zou C., Fu Y.,Xie Q. and Yao S., High-performance glucose amperometric biosensor based on magnetic polymeric bionanocomposites,Biosensors & Bioelectronics, 2010,25, 1277-1282.

六、依托单位给予的支持

学校按照教育部重点实验室建设要求和管理条例，在人力、物力和财力方面对“化学生物学及中药分析”重点实验室给予全力支持,学校每年20万元的运行经费。

七、运行经费、主任基金等的使用情况

重点实验室运行经费主要分为主任基金、开放基金、学术交流经费、办公经费四个方面，大致比例为主任基金10%、开放基金40%、学术交流经费40%、办公经费10%。主任基金主要用于支持具有创新思想的课题和优秀年轻人才的培养。在符合国家有关政策的前提下，可用于岗位补贴、绩效奖励等。开放基金主要用于吸引和资助国内外优秀学者，特别是青年学者，来实验室开展合作研究，培养造就高层次创新人才，促进交叉学科方向的形成和发展，提高科研水平。学术交流经费主要用于学术委员会会议的开支，以及补贴邀请校外专家接待费、差旅费等开支。办公经费主要用于购置办公设备、网站维护和建设、改善实验室人员办公条件和实验室日常管理等开支。

2010年学校拨运行经费20万，主要用于开放基金，仪器设备费、材料与资料费、会议差旅费、维修费、学术交流、复印与打印等方面。

2009年度的开放基金申请评审工作已于2008年11月份经化学生物学及中药分析教育部重点实验室学术委员会审批，汪保和等来自湖南师范大学的12位老师将获得资助，2010年经费落实，具体情况见下表。

另外，通过重点学科和湖南省拨给姚院士的经费，投入实验室购置仪器设备，具体如下: